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MicroscoPi
- Open-source microscopy platform with automated xyz control
- Low cost construction with readily available components and 3d-printed frame
- Easy to use, browser-based GUI that can be run from any internet capable device
- Designed for teaching, outreach and field microscopy
Multi-functional + democratised = microscopi
Automated XYZ stage control
Fully motorised stage
Open-source software
Using a web browser based GUI, users can fully control the stage and camera via their phone or tablet. Acquired images are saved onto the user's device, making Microscopi a perfect tool for engaging students and non-specialists.
Accessible
With 3D-printed or inexpensive off-the-shelf components, Microscopi offers the functionality of a complex system that is much more affordable.
Multi-functional
Through using an LED array for condenser-free illumination, Microscopi can achieve a range of imaging modalities including bright-field, fluorescence, dark-field and a phase contrast equivalent.
A new way to approach Imaging
A Democratised Automated Imaging System
For many people the idea of microscopy involves a user sitting at a traditional microscope, looking down the eyepieces while focussing and moving the stage. In leading research institutes, advanced microscopy involves the use of sophisticated imaging systems that utilise automated stages that are capable of multidimensional imaging. Allied to acquisition software and cameras this allows the researcher to obtain data in XYZ as well as taking time lapse images. Due to the high cost, size and complicated mode of using these systems it is rare that those involved in outreach, teaching, field-work or even many involved in scientific research can use commercial microscopes.
Microscopi is a small and portable automated imaging system that can allow scientists without the resources for commercial systems to access a set up with stage and camera control. The 3D-printed and readily available components also mean the construction of the system can be used as an imaging, electronics and engineering project for students. This will allow them to really understand how the system works and also means maintenance by the end user can avoid costly service contracts associated with more sophisticated setups. Illumination with an LED array allows close control of light to give bright-field, dark-field and psuedo-phase contrast without the use of a condenser lens.
Development Team
Dr. Matthew Wincott
Dr. Andrew Jefferson
Dr. Matthew Wincott
Lead Scientist/Engineer
Matthew designed and built the Microscopi. He implemented the Python-based control system and web GUI, enabling complete control of Microscopi from mobile devices. Together with Richard, the design was refined for 3d printing.
Dr. Richard Parton
Dr. Andrew Jefferson
Dr. Matthew Wincott
Senior Scientist - Micron & Biochemistry
As the imaging expert on the project he drove the design and development alongside Matthew. Richard devised central components of the system, including the z-lifter for the stage. Richard also co-developed and implemented the different imaging modes in the system, contributing to the designs of the
Senior Scientist - Micron & Biochemistry
As the imaging expert on the project he drove the design and development alongside Matthew. Richard devised central components of the system, including the z-lifter for the stage. Richard also co-developed and implemented the different imaging modes in the system, contributing to the designs of the fluorescent light path as well as the illumination array.
Dr. Andrew Jefferson
Dr. Andrew Jefferson
Dr. Andrew Jefferson
Test Scientist – Micron & Biochemistry
Andrew tested the Microscopi at varying stages of development and provided Matthew and Richard with feedback. He also assisted with component research, procurement and assembly.
Prof. Martin Booth
Prof. Martin Booth
Prof. Martin Booth
Principal Investigator - Engineering
Martin was the engineering consultant on the design process from the initial stages. As Matthew's manager he had key intellectual input throughout the project.
Dr. Ian Dobbie
Prof. Martin Booth
Prof. Martin Booth
Senior Consulting Scientist - Micron & Biochemistry
Ian worked with Ilan, Richard and Matthew to move from the proof of concept to working versions. He provided a key link between the engineering and imaging aspects of the project.
Prof. Ilan Davis
Prof. Martin Booth
Prof. Ilan Davis
Principal Investigator - Micron & Biochemistry
Ilan was the initiator of the project, having put together a simple prototype as a proof of concept. Utilising the expertise within the Micron Imaging Facility, he managed the development of the Microscopi project.
Teaching with Microscopi
Construction Project
Imaging from your Garden
Imaging from your Garden
Microscopi is an ideal medium time frame project for students to learn 3D-printing, electronics, coding and simple optics.
Imaging from your Garden
Imaging from your Garden
Imaging from your Garden
Take samples of plants, animals or even just sand from your garden; skim your pond for creepy crawlies then use your phone to drive Microscopi and save images and movies to your phone.
Microscopy
Microscopy
Microscopy
See the effect of different illumination modes on samples, set up time lapse and z-stack projects, explore the limits of resolution and so much more.
Coding
Microscopy
Microscopy
Work with our open source GUI to learn code that has practical outcomes. From changing the appearance of the GUI to writing code to control more complex stage movements and illumination control.