Micron BioImaging Facility
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Micron BioImaging Facility

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  • Home
  • About us
    • About the Facility
    • Mission Statement
    • Acknowledgement Policy
    • Alumni
  • Our Technology
    • Microscope Systems
    • Image Analysis
    • OMERO
  • Research
    • Publications
    • Microscope Development
    • Software Development
  • Access
    • FAQs
    • COVID-19 Guidelines
    • Booking
  • Education and Outreach
    • Training Courses
    • SIM Practical Course 2022
    • Microscopi 3D-printed
  • News and Events
  • Image Gallery

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MicroscoPi

  • Open-source microscopy platform with automated xyz control
  • Low cost construction with readily available components and 3d-printed frame
  • Easy to use, browser-based GUI that can be run from any internet capable device
  • Designed for teaching, outreach and field microscopy

Biorxiv pre-print

Multi-functional + democratised = microscopi

Automated XYZ stage control

Fully motorised stage

Open-source software

Using a web browser based GUI, users can fully control the stage and camera via their phone or tablet. Acquired images are saved onto the user's device, making Microscopi a perfect tool for engaging students and non-specialists.

Accessible

With 3D-printed or inexpensive off-the-shelf components, Microscopi offers the functionality of a complex system that is much more affordable. 

Multi-functional

Through using an LED array for condenser-free illumination, Microscopi can achieve a range of imaging modalities including bright-field, fluorescence, dark-field and a phase contrast equivalent. 

Sample Images

    A new way to approach Imaging

    A Democratised Automated Imaging System

    For many people the idea of microscopy involves a user sitting at a traditional microscope, looking down the eyepieces while focussing and moving the stage.  In leading research institutes, advanced microscopy involves the use of sophisticated imaging systems that utilise automated stages that are capable of multidimensional imaging. Allied to acquisition software and cameras this allows the researcher to obtain data in XYZ as well as taking time lapse images. Due to the high cost, size and complicated mode of using these systems it is rare that those involved in outreach, teaching, field-work or even many involved in scientific research can use commercial microscopes. 


    Microscopi is a small and portable automated imaging system that can allow scientists without the resources for commercial systems to access a set up with stage and camera control. The 3D-printed and readily available components also mean the construction of the system can be used as an imaging, electronics and engineering project for students. This will allow them to really understand how the system works and also means maintenance by the end user can avoid costly service contracts associated with more sophisticated setups. Illumination with an LED array allows close control of light to give bright-field, dark-field and psuedo-phase contrast without the use of a condenser lens. 



    Sample Videos

    A water flea head imaged on Microscopi

    User Instructions

    User Manual - Microscopi (pdf)

    Download

    Assembly Instructions (pdf)

    Download

    Ways to Use Microscopi

    Teaching

    Scientific Outreach

    Scientific Outreach

    Either by building a unit or using one for imaging projects, Microscopi is an excellent teaching and learning tool

    Coming Soon

    Scientific Outreach

    Scientific Outreach

    Scientific Outreach

    Engage younger audiences and non-experts with an intuitive system they can control on their phone or on a tablet. They can drive the system and take and save their images using only a web browser.

    Coming Soon

    Fieldwork

    Scientific Outreach

    Fieldwork

    Bring a fluorescent microscope that can easily image even large samples to the field that works from batteries while contained in its own 3D-printed case. 

    Coming Soon

    Development Team

    Dr. Matthew Wincott

    Dr. Andrew Jefferson

    Dr. Matthew Wincott

    Lead Scientist/Engineer

    Matthew designed and built the Microscopi. He implemented the Python-based control system and web GUI, enabling complete control of Microscopi from mobile devices. Together with Richard, the design was refined for 3d printing.

    Dr. Richard Parton

    Dr. Andrew Jefferson

    Dr. Matthew Wincott

    Senior Scientist - Micron & Biochemistry

    As the imaging expert on the project he drove the design and development alongside Matthew. Richard devised central components of the  system, including the z-lifter for the stage. Richard also co-developed and implemented the different imaging modes in the system, contributing to the designs of the

    Senior Scientist - Micron & Biochemistry

    As the imaging expert on the project he drove the design and development alongside Matthew. Richard devised central components of the  system, including the z-lifter for the stage. Richard also co-developed and implemented the different imaging modes in the system, contributing to the designs of the fluorescent light path as well as the illumination array. 


    Test Scientist – Micron & Biochemistry

    Dr. Andrew Jefferson

    Dr. Andrew Jefferson

    Dr. Andrew Jefferson

    Test Scientist – Micron & Biochemistry

    Andrew tested the Microscopi at varying stages of development and provided Matthew and Richard with feedback. He also assisted with component research, procurement and assembly.

    Prof. Martin Booth

    Prof. Martin Booth

    Prof. Martin Booth

    Principal Investigator - Engineering


    Martin was the engineering consultant on the design process from the initial stages. As Matthew's manager he had key intellectual input throughout the project.



    Dr. Ian Dobbie

    Prof. Martin Booth

    Prof. Martin Booth

    Senior Consulting Scientist - Micron & Biochemistry


    Ian worked with Ilan, Richard and Matthew to move from the proof of concept to working versions. He provided a key link between the engineering and imaging aspects of the project.

    Prof. Ilan Davis

    Prof. Martin Booth

    Prof. Ilan Davis

    Principal Investigator - Micron & Biochemistry


    Ilan was the initiator of the project, having put together a simple prototype as a proof of concept. Utilising the expertise within the Micron Imaging Facility, he managed the development of the Microscopi project. 

    Teaching with Microscopi

    Construction Project

    Imaging from your Garden

    Imaging from your Garden

    Microscopi is an ideal medium time frame project for students to learn 3D-printing, electronics, coding and simple optics. 

    Imaging from your Garden

    Imaging from your Garden

    Imaging from your Garden

    Take samples of plants, animals or even just sand from your garden; skim your pond for creepy crawlies then use your phone to drive Microscopi and save images and movies to your phone.

    Microscopy

    Microscopy

    Microscopy

    See the effect of different illumination modes on samples, set up time lapse and z-stack projects, explore the limits of resolution and so much more.

    Coding

    Microscopy

    Microscopy

    Work with our open source GUI to learn code that has practical outcomes. From changing the appearance of the GUI to writing code to control more complex stage movements and illumination control. 

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