Fully motorised stage
Using a web browser based GUI, users can fully control the stage and camera via their phone or tablet. Acquired images are saved onto the user's device, making Microscopi a perfect tool for engaging students and non-specialists.
With 3D-printed or inexpensive off-the-shelf components, Microscopi offers the functionality of a complex system that is much more affordable.
Through using an LED array for condenser-free illumination, Microscopi can achieve a range of imaging modalities including bright-field, fluorescence, dark-field and a phase contrast equivalent.
For many people the idea of microscopy involves a user sitting at a traditional microscope, looking down the eyepieces while focussing and moving the stage. In leading research institutes, advanced microscopy involves the use of sophisticated imaging systems that utilise automated stages that are capable of multidimensional imaging. Allied to acquisition software and cameras this allows the researcher to obtain data in XYZ as well as taking time lapse images. Due to the high cost, size and complicated mode of using these systems it is rare that those involved in outreach, teaching, field-work or even many involved in scientific research can use commercial microscopes.
Microscopi is a small and portable automated imaging system that can allow scientists without the resources for commercial systems to access a set up with stage and camera control. The 3D-printed and readily available components also mean the construction of the system can be used as an imaging, electronics and engineering project for students. This will allow them to really understand how the system works and also means maintenance by the end user can avoid costly service contracts associated with more sophisticated setups. Illumination with an LED array allows close control of light to give bright-field, dark-field and psuedo-phase contrast without the use of a condenser lens.
A water flea head imaged on Microscopi
Either by building a unit or using one for imaging projects, Microscopi is an excellent teaching and learning tool
Engage younger audiences and non-experts with an intuitive system they can control on their phone or on a tablet. They can drive the system and take and save their images using only a web browser.
Bring a fluorescent microscope that can easily image even large samples to the field that works from batteries while contained in its own 3D-printed case.
Matthew designed and built the Microscopi. He implemented the Python-based control system and web GUI, enabling complete control of Microscopi from mobile devices. Together with Richard, the design was refined for 3d printing.
Senior Scientist - Micron & Biochemistry
As the imaging expert on the project he drove the design and development alongside Matthew. Richard devised central components of the system, including the z-lifter for the stage. Richard also co-developed and implemented the different imaging modes in the system, contributing to the designs of the
Senior Scientist - Micron & Biochemistry
As the imaging expert on the project he drove the design and development alongside Matthew. Richard devised central components of the system, including the z-lifter for the stage. Richard also co-developed and implemented the different imaging modes in the system, contributing to the designs of the fluorescent light path as well as the illumination array.
Test Scientist – Micron & Biochemistry
Andrew tested the Microscopi at varying stages of development and provided Matthew and Richard with feedback. He also assisted with component research, procurement and assembly.
Principal Investigator - Engineering
Martin was the engineering consultant on the design process from the initial stages. As Matthew's manager he had key intellectual input throughout the project.
Senior Consulting Scientist - Micron & Biochemistry
Ian worked with Ilan, Richard and Matthew to move from the proof of concept to working versions. He provided a key link between the engineering and imaging aspects of the project.
Principal Investigator - Micron & Biochemistry
Ilan was the initiator of the project, having put together a simple prototype as a proof of concept. Utilising the expertise within the Micron Imaging Facility, he managed the development of the Microscopi project.
Microscopi is an ideal medium time frame project for students to learn 3D-printing, electronics, coding and simple optics.
Take samples of plants, animals or even just sand from your garden; skim your pond for creepy crawlies then use your phone to drive Microscopi and save images and movies to your phone.
See the effect of different illumination modes on samples, set up time lapse and z-stack projects, explore the limits of resolution and so much more.
Work with our open source GUI to learn code that has practical outcomes. From changing the appearance of the GUI to writing code to control more complex stage movements and illumination control.
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