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Microscopy Hardware Development at Micron

DeepSIM - Upright structured illumination with AO

DeepSIM is the first upright 3D-SIM microscope in the world, uniquely allowing samples to be imaged at super-resolution while manipulating them for electrophysiological measurements and/or micro-injection. Rather than moving the specimen or the objective for taking Z stacks, adaptive optics are used instead. An SLM will be used for rapid production of stripes for live 3D-SIM and for additional aberration corrections.The adaptive optics will allow us to extend the depth of 3D-SIM far beyond what is possible on commercial instruments. The ability to image deep (~50 microns) while manipulating the specimen will be transformational for many biological projects. 


Team Members: Ian Dobbie, Antonia Göhler, Mick Phillips, Mantas Zaurukas, Nick Hall, Josh Titlow, Richard Parton.

4Pi-SMS single molecule interferometric microscope

A replica of a revolutionary new microscope platform with outstanding resolution, using two opposing objectives.  The microscope was designed by a consortium at Yale lead by Jim Rothman and funded by a Strategic Award from Wellcome. The design is a new implementation of iPALM/4Pi-SMSN, termed whole-cell 4Pi single-molecule switching nanoscopy (W-4PiSMSN), which extends the imaging capabilities of this technology to whole cells without compromising resolution. A key design feature is the use of adaptive optics technology lead by Martin Booth. 


Team Members: Mick Phillips, Ian Dobbie and Martin Booth

MicroscoPi

The vision of Ilan Davis, this  project aims to develop a low-cost open source microscopy platform for teaching and outreach. The system being developed uses a low-cost microprocessor, the Raspberry Pi, to interface with hardware and provide a user-friendly microscope interface. The project is developed with a strong focus on designing the microscope as a learning tool for microscopy and programming. To this end, the microscope will be low-cost, easily assembled, robust, and include many elements that can be fabricated using a 3d printer. This project has been headed up by Matthew Wincott who is working in collaboration with us in the Engineering dept.


Team Members: Matthew Wincott; Richard M Parton; Ian Dobbie; Martin Booth; Ilan Davis

Cryo-3D-SIM

Based on an OMX-T design in collaboration with John Sedat (UCSF), we have constructed the first Cryo-3D-SIM/STORM microscope. It allows 3D-SIM on cryo preserved samples at liquid nitrogen temperatures, which sharpens the fluorescence emission peaks, increases photo stability and allows imaging of cryo-preserved specimens that have not been distorted by chemical fixation. We have demonstrated this system uisng cryo-DSTORM (See Kaufmann et al., 2014 - Nano Letters) and have a working system that is heavily used for correlative fluorescence and soft X-ray work at the Diamond Light Source at Harwell (www.diamond.ac.uk/Home.html) alongside the Beamline 24 soft-X-ray microscope. We working on the next generation instrument that will use  adaptive optics correct aberrations and focus remotely and enable cryo-saturated-SIM, which will double the resolution again beyond conventional 3D-SIM.