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Micron BioImaging Facility
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Zeiss LSM 980 with Airyscan2

 Zeiss LSM 880 has been replaced in December 2020 for the newest model LSM980 with Airyscan2. LSM980 is an upright laser scanning confocal with Airyscan2 technology, located in the 00-060 imaging room.
 

The Airyscan module is a new detector concept featuring a 32 channel GaAsP-PMT area detector. The Airyscan detector brings simultaneous improvements in spatial resolution and signal-to-noise ratio in comparison with conventional confocal microscopy. The 1.5X improvement in lateral resolution of the Airyscan technology allows the LSM 880 microscope to resolve small structures up to 120 nm in XY and 350 nm in Z. 


The LSM980 uses Zen Blue for data acquisition and it is equipped with a much better and easy to use Tiles and Positions, for multipoint visiting, tiling and stitching. See below the videos from training  session with Zeiss, covering the biggest changes between LSM880 and LSM980, to assist the transition of existing users. 


For support, new users access, training and booking please contact micron@bioch.ox.ac.uk.


Applications

  • Conventional and super-resolution confocal microscopy on live and fixed cells, tissues, engineered constructs and non-biological materials
  • Multi-channel XYZ fluorescence and transmitted light imaging modalities
  • Optical sectioning for 3D imaging
  • Live cell imaging, zebra fish, any imaging requiring top down approach into buffer or culture media with dipping objectives
  • Spectral imaging
  • Temperature environment control 
  • Time-lapse imaging
  • Tile scanning and stiching 
  • FRET, FRAP, FCS, colocalisation

Technical specifications

Objectives

Light sources and Detectors

Light sources and Detectors

Light sources and Detectors

Light sources and Detectors

Light sources and Detectors

Additional Information

Zeiss_880_User_guide (pdf)Download
Airyscanning Novel Approach to Confocal Imaging (pdf)Download
LSM-880_Basic-Principle-Airyscan (pdf)Download
Fast mode Zeiss LSM 880 with Airyscan (pdf)Download

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