Equipment

The LSM 980 is an upright laser scanning confocal microscope equipped with Airyscan 2 technology. The Airyscan detector delivers simultaneous improvements in spatial resolution and signal-to-noise ratio compared with conventional confocal microscopy. With a 1.5× improvement in lateral resolution, Airyscan technology enables the LSM 980 to resolve structures down to approximately 120 nm in the XY plane and 350 nm in Z. The system uses ZEN Blue software for data acquisition and includes the Tiles and Positions module for multi-point imaging, tiling, and stitching.

The microscope is well suited for multi-channel volumetric imaging of cells and tissues, offering near super-resolution performance in Airyscan 2 mode. It is also equipped with dipping objectives for imaging fluorescent cellular structures such as spheroids and organoids. In addition, Fluorescence Recovery After Photobleaching (FRAP) and Fluorescence Correlation Spectroscopy (FCS) capabilities enable quantitative analysis of molecular dynamics, concentration, and interactions.

Automated widefield & SD microscope capable of high-content screening of multi-colour fluorescently labelled cell structures and populations. The ScanR system excels in drug discovery applications, including showing the biochemical effects of compounds on the cellular level and drug-induced changes at gene expression levels. 

Covering a wide range of applications including protein localisation and colocalisation, intracellular transport, automatic FISH analysis and rare event analysis for live cell imaging. The ScanR is also equipped with a DP23 colour camera, making it ideal for capturing high-resolution images of histology samples or naturally pigmented specimens.

These single molecule microscopes can be used to identify the position of single fluorescent protein and/or lipid molecules inside a cell. The molecule positions can then be used to extract information on molecular organisation at a higher level. 

The microscope operates in HILO or in 3D mode. Single Molecule Localisation Microscopy (SMLM) capabilities include direct STochastic Optical Reconstruction Microscopy (dSTORM), Photoactivation Localisation Microscopy (PALM) and DNA points accumulation for imaging in nanoscale topography (DNAPAINT). The microscope also allows for SPT in HILO.

The iSIM (instant structured illumination microscopy) microscope is integrated into a Evident microscope platform. It combines ultra-fast 3D imaging with low phototoxicity and provides close to a two-fold resolution enhancement compared to standard confocal or widefield fluorescence microscopy. The system is equipped for live cell super-resolution imaging with twin Hamamatsu Orca Quest qCMOS cameras.

The new Stellaris TauSTED with FALCON is the latest in cutting edge super-resolution technology from Leica. The system is equipped for STED, Spectraplex, fluorescence lifetime (FALCON) and confocal microscopy. 

TauSTED Xtend is the next step in the evolution of STED imaging. It combines lifetime-based information with an additional spatial readout. This results in a significant gain in resolution, especially at lower STED depletion powers (589 and 775 nm), allowing to extend multi-colour live imaging capabilities at the nanoscale.

Structured Illumination Microscopy (SIM) allows the user to observe living and fixed fluorescent samples at resolution greater than a standard fluorescence microscope. Imaging at this resolution can reveal structural organisation inside a cell that can be otherwise masked. 

Equipped with three cameras the microscope enables true simultaneous imaging of three channels. Modalities available include 2D SIM, 3D SIM, TIRF SIM and ring-TIRF. Three colour simultaneous single particle tracking (SPT) and localisation is also possible using the ring-TIRF configuration. The system is equipped for live cell imaging.

A high-speed, widefield microscope with LED excitation from 390 to 747 nm for live and fixed sample imaging. The Leica THUNDER enables you to obtain a clear view of cellular details, even deep within an intact sample, in real time without out-of-focus blur. 

THUNDER brings you high-speed, multi-colour imaging of thin and thick samples with increased temporal resolution.