Micron Advanced Microscopy Course 2020

We are very excited to be able to run our very popular Micron Advanced Microscopy Course this year. We have revised the course content and arranged to run the course virtually, including the demos and practicals. The course is open to Medical Sciences Division (MSD) postgraduate research students and Oxford research community.  Registration is now open on the MSD skills website. 

 The main objective of the course is :

• Understand the theoretical and practical aspects of optical microscopy.
• Understand the different imaging modalities and its applications in biomedical research. 
• Learn about image data handling, image analysis and appreciate the significance of AI. 
• Understand the design and built considerations of bespoke microscopes. 

Course layout:

Day 1: Principles of light microscopy
Day 2: Fluorescence microscopy
Day 3: Quantitative bioimaging
Day 4: Super- resolution microscopy
Day 5: Demos and Practicals

Have a look at the 2020 course programme HERE.  We look forward to seeing you all! 

This course is aimed at facility staff working with the DeltaVision OMX 3D structured illumination microscope (3D-SIM). The hands-on course covered a whole range of topics, theory, instrument design and details, calibration, sample preparation, reconstructions and data quality checks. The main speakers/tutors were Marcel Müller, Ian Dobbie and Lothar Schermelleh. 

Course material: 

1. Introduction
2. SIM theory and data reconstruction - Marcel Müller
3. Implementing SIM in practice - Ian Dobbie
4. 3D - SIM and reconstruction artefacts - Lothar Schermelleh 

Micron runs a course in November every year aimed at researchers and students interested in learning both theoretical and practical aspects of optical microscopy. The course introduces core principles of brightfield and fluorescence microscopy, biological applications of different imaging approaches and super-resolution techniques. 

Course content: 

• General introduction to light microscopy and optics
• Principles of microscopy and microscope anatomy
• Detectors for imaging
• Contrast enhancement (phase contrast and DIC)
• Understanding fluorescence microscopy
• Fluorescent dyes and proteins
• Fundamentals of Microscope Image Analysis
• Handling of Microscope Image Data
• Confocal Microscopy
• Live-cell Imaging
• Imaging at the molecular level: F-Techniques 
• Microscope Image Analysis 
• Light sheet fluorescence microscopy  
• Super resolution and SIM
• Nanometer resolution by localisation microscopy
• STED/STED-FCS
• Bespoke microscope design
• Summary

Course material from previous year can be downloaded here. Everyone is welcome to use the teaching material, but we would appreciate an acknowledgement of the authors and Micron Oxford as the source.