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Background
On Sept 12th the Micron Bioimaging Facility are hosting a one day single molecule workshop in collaboration with ONI.
Lectures will cover topics such as molecular cluster analysis, DNA-PAINT, deep learning, 2D and 3D single molecule localisation and single particle tracking. The workshop will be especially valuable to anyone new to the field of single molecule imaging.
Programme
The workshop will feature morning talks from some of the leaders in the field of single molecule imaging as well as ECRs applying SM in their research.
Confirmed speakers include:
- Susan Cox, King’s College London
- Franky Djutanta, ONI
- Rebecca Saleeb, University of Edinburgh
- Daniel Nieves, University of Birmingham
- Kazuhiro Maeshima, National Institute of Genetics Japan
- Jingyu Wang, Department of Engineering, Oxford
- Amy Moores, Department of Biochemistry, Oxford
- Christof Hepp, Department of Physics, Oxford
- Flavia Constantinescu, Department of Biochemistry, Oxford
Following lunch there will be a speed networking session allowing you the opportunity to meet other scientists interested in single molecule imaging.
The remainder of the day will be spent in small group demonstrations with the ONI Nanoimager. There will also be a Tips and Tricks session on sample prep for single molecule imaging.
Practical 1: Using the Nanoimager for two-colour Single Molecule Localisation Microscopy (SMLM).
This practical will focus on using the Nanoimager system for SMLM. We will provide an overview of the Nanoimager and its applications in SMLM. 2D and 3D single molecule acquisition and analysis will be demonstrated. SMLM circumvents the diffraction limit, allowing researchers to visualise biological structures, subcomplexes and proteins with a resolution greater than 20 nm, with ten times better detail than with conventional fluorescence techniques.
Practical 2: A demonstration of single-particle tracking carried out on the Nanoimager.
This practical will introduce the technique of multi-colour single particle tracking. Single-particle tracking (SPT) is a powerful tool in microscopy that allows the motion of individual fluorescently labelled particles (molecules, vesicles, virions or other molecular complexes) to be followed within a medium or in living cells and to obtain information on their dynamic behaviour over time. Single-particle tracks can help scientists better understand the positions, paths and interactions of molecules in systems that are highly dynamic or require imaging over extended periods of time.
Registration
Half day attendance includes morning talks with coffee/tea and the networking lunch - £55.00 Academic Rate and £110.00 Industry Rate.
Full day attendance includes talks with coffee/tea, networking lunch and 2 practical workshops - £95.00 Academic Rate and £190.00 Industry Rate. The workshop will run from 9.30 to 17.00.
Payment details will be sent to attendees in advance of the workshop. The academic workshop fee is VAT exempt.
Please note that places for the Nanoimager demonstrations are limited, therefore please register early if you wish to attend these.
Registration has now closed for the full day workshop, if you would like to attend for the half day workshop please register using this link
Cancellation policy
The full registration fee is payable in advance. Cancellations should be done in writing to micron@bioch.ox.ac.uk. There is a 10% charge for cancellations received before the 5th of September 2023. No refunds will be made for cancellations received after the 5th of September for the inability to attend the course for whatever reasons. The University is not liable for non-attendance due to travel disruptions, health problems or any other reason that might lead to a delegate not being able to attend the course. Attendees are strongly advised to have appropriate insurance.