Deltavision OMX SR

Super-resolution microscopy allows the user to observe living and fixed fluorescent samples at  a resolution greater than a standard fluorescence microscope. Imaging at this resolution can reveal structural organisation inside a cell that can be otherwise masked. 

The OMX SR is an inverted wide-field microscope. Equipped with three cameras the microscope enables true simultaneous imaging of three channels. Modalities available include 2D SIM, 3D SIM, TIRF SIM, ringTIRF , FRAP and DIC. Three colour simultaneous single particle tracking (SPT) and localisation is also possible using the ring-TIRF configuration. The system is fully equipped for live cell imaging.

Lasers lines: 405 nm, 488 nm, 561 nm and 639 nm

Objectives:  Olympus 60x Oil 1.5 NA TIRF UPLAPO, Olympus 60x 1.30 NA Silicone UPLANSApo

Cameras: 3 x sCMOS PCO Edge 4.2

Environmental control: temperature and gas, carbon dioxide and nitrogen (hypoxia studies)

Mamou, G., Corona, F., Cohen-Khait, R. et al. Peptidoglycan maturation controls outer membrane protein assembly. Nature 606, 953–959 (2022). https://doi.org/10.1038/s41586-022-04834-7

Miron, E., Windo, J., Ochs, F., Schermelleh, L. (2022). Replication Labeling Methods for Super-Resolution Imaging of Chromosome Territories and Chromatin Domains. In: Leake, M.C. (eds) Chromosome Architecture. Methods in Molecular Biology, vol 2476. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2221-6_9

Vergara HM, Pape C, Meechan KI. et al. Whole-body integration of gene expression and single-cell morphology. Cell, (2021). doi: 10.1016/j.cell.2021.07.017.