Micron BioImaging Facility
Micron BioImaging Facility
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    • SIM Practical Course 2022
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    • Home
    • About us
      • About the Facility
      • Mission Statement
      • Acknowledgement Policy
      • Alumni
    • Our Technology
      • Microscope Systems
      • Image Analysis
    • Access
      • FAQs
      • Booking
    • Education and Outreach
      • Training Courses
      • SIM Practical Course 2022
      • SM Workshop 2023
    • Research
      • Publications
      • Microscope Development
      • Software Development
    • News and Events
    • Image Gallery
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  • Home
  • About us
    • About the Facility
    • Mission Statement
    • Acknowledgement Policy
    • Alumni
  • Our Technology
    • Microscope Systems
    • Image Analysis
  • Access
    • FAQs
    • Booking
  • Education and Outreach
    • Training Courses
    • SIM Practical Course 2022
    • SM Workshop 2023
  • Research
    • Publications
    • Microscope Development
    • Software Development
  • News and Events
  • Image Gallery

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Our Training Courses

The Micron Bioimaging Facility host a number of microscopy courses and workshops every year. Courses are open to all from both industry and academia.  Bespoke microscopy courses are available on request. If you would like more details of our courses or would like to be included on our mailing list please email us via micron@bioch.ox.ac.uk

Micron Short Courses in Microscopy Series

The Micron short course curriculum provides an in-depth study of microscopy, from the basic principles to advanced super-resolution methods. These theoretical courses are intended to complement the practical and experimental training provided during your practical microscopy training session at Micron. Participation in these courses is strongly encouraged for anyone utilising microscopy in their research. 


Micron users can book a place on each course via PPMS. New users should contact micron@bioch.ox.ac.uk for registration. 


Hilary 2025 course dates are Feb 12th, 13th and 20th. These courses are small group sessions with a max of 6 to 8 attendees per session. The cost per course is £55.00.

PPMS course registration

Introduction to fluorescence microscopy

This introductory course is designed to give you a foundational understanding of fluorescence microscopy. The following key topics will be covered:


  • Using fluorescence microscopy to understand the inner workings of biology.
  • Specificity and contrast in microscopy. 
  • Techniques in quantitative fluorescence microscopy.
  • Overview of various types of fluorescence microscopes. 
  • Tags: small molecules, antibodies, nanobodies, GFP, Halo, FISH.  
  •  Core principles of fluorescence and Jablonski diagrams. 
  • Understanding fluorescent spectra, fluorophores, and autofluorescence.
  • Addressing challenges in fluorescence microscopy: photobleaching and phototoxicity.


No prior experience with fluorescence microscopy is required for this course.

Confocal and widefield microscopy

This course is designed to provide you with an in-depth understanding of the two fundamental microscopy techniques: widefield and confocal microscopy. The following subjects will be covered:


Anatomy of a Fluorescence Microscope: Explore the key components of a fluorescence microscope, including objectives, illumination sources, dichroic mirrors, and detectors.


Widefield vs. Confocal Microscopy: Understand the differences between Widefield and Confocal microscopy and learn how to choose the appropriate technique for your research project.


Magnification and Resolution Concepts: Magnification and resolution, with an introduction to Abbe’s resolution limit, the Rayleigh Criterion, and Nyquist sampling.


Ideal vs. Real Microscopy: the differences between ideal and real microscopy, covering topics such as spherical and chromatic aberrations, scattering, background noise, and the importance of deconvolution in image processing.

Sample preparation for fluorescence microscopy

Sample preparation has a major impact on microscopy and good sample preparation is a prerequisite to ensure high quality data is captured. In this course we will cover the multiple steps of sample preparation for fluorescence microscopy, including the imaging workflow, fixation, permeabilisation, fluorescence labelling and staining, and choosing fluorophores. Understanding the impact of each step in the sample preparation process is essential for achieving optimal imaging results

Introduction to super-resolution microscopy

This course delves into the theory and application of super-resolution microscopy. It is designed for participants who have an immediate need to implement these advanced techniques in their biological research. The course will cover the following topics: STED, SIM, localization microscopy (including PALM, dSTORM, and PAINT), pixel-reassignment, and super-resolution imaging of living samples. All these super-resolution techniques are available to access in Micron. 


Participants should have a solid understanding of basic microscopy techniques and some experience in fluorescence microscopy. Familiarity with biological imaging and prior exposure to advanced imaging methods will be beneficial, as the course is geared towards those who plan to apply super-resolution to their research.

Tips and Tricks for SMLM

This course delivers tips and tricks for those starting out in SMLM. The course covers the applications of SMLM,  sample preparation, types of hardware, image acquisition and an introduction the FIJI Plug-in ThunderSTORM. 

PPMS course registration

Charge Policy

The registration fee is £55 per course and this will be charged at month end for University of 

Oxford staff and students. Course cancellations within 48 hours will be charged at the full rate. These courses run a waiting list, therefore if you can no longer attend a course please contact us as soon as possible.  Substitutions may made be please inform us via micron@bioch.ox.ac.uk

PPMS course registration

Single Molecule Workshop 2023

Single Molecule Workshop 2023

Single Molecule Workshop 2023

September 12th 2023

Learn more

SIM Practical Course 2022

Single Molecule Workshop 2023

Single Molecule Workshop 2023

Super-resolution microscopy course

Learn more

Advanced Microscopy 2020

Single Molecule Workshop 2023

Advanced Microscopy 2020

Introductory 

microscopy course

Learn more

OMX SIM Course 2019

Single Molecule Workshop 2023

Advanced Microscopy 2020

Super-resolution microscopy course

Learn more



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