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Super-resolution development
4Pi-SMS microscope
Upright DeepSIM with AO
4Pi-SMS microscope
The 4PI-SMS system uses a set-up of two opposing lenses and adaptive optics to enable super-resolution 3D localisation microscopy of entire cell volumes with an isotropic resolution of 10-20 nm in x, y and z-directions. Multi-colour imaging is achieved by simultaneously detecting two-three spectrally similar dyes (e.g. AF647/CF660 or CF68
The 4PI-SMS system uses a set-up of two opposing lenses and adaptive optics to enable super-resolution 3D localisation microscopy of entire cell volumes with an isotropic resolution of 10-20 nm in x, y and z-directions. Multi-colour imaging is achieved by simultaneously detecting two-three spectrally similar dyes (e.g. AF647/CF660 or CF680) on the same two cameras separated by carefully selected dichroic filters.
The prototype of this revolutionary microscope platform was first published in Cell by Huang et al., in 2016 lead by Joerg Bewersdorf and Jim Rothman from Yale University. This instrument at Oxford is funded by a Strategic Award from the Wellcome Trust. It is fully operational and generates excellent 3D single molecule data.
If you are interested in accessing the 4PI-SMS, please fill our expression of interest form below.
This microscope was developed by at Micron by Jingyu Wang, Mick Phillips, Ian Dobbie and Martin Booth
Cryo-3D-SIM
Upright DeepSIM with AO
4Pi-SMS microscope
Based on an OMX-T design in collaboration with John Sedat (UCSF), we have constructed the first Cryo-3D-SIM/STORM microscope. It allows 3D-SIM on cryo preserved samples at liquid nitrogen temperatures, which sharpens the fluorescence emission peaks, increases photo stability and allows imaging of cryo-preserved specimens that have not bee
Based on an OMX-T design in collaboration with John Sedat (UCSF), we have constructed the first Cryo-3D-SIM/STORM microscope. It allows 3D-SIM on cryo preserved samples at liquid nitrogen temperatures, which sharpens the fluorescence emission peaks, increases photo stability and allows imaging of cryo-preserved specimens that have not been distorted by chemical fixation. We have demonstrated this system uisng cryo-DSTORM (See Kaufmann et al., 2014 - Nano Letters) and have a working system that is heavily used for correlative fluorescence and soft X-ray work at the Diamond Light Source at Harwell (www.diamond.ac.uk/Home.html) alongside the Beamline 24 soft-X-ray microscope. We working on the next generation instrument that will use adaptive optics correct aberrations and focus remotely and enable cryo-saturated-SIM, which will double the resolution again beyond conventional 3D-SIM.
If you are interested in accessing the Cryo-3D-SIM, please fill our expression of interest form below.
Upright DeepSIM with AO
Upright DeepSIM with AO
Upright DeepSIM with AO
DeepSIM is the first upright 3D-SIM microscope in the world, uniquely allowing samples to be imaged at super-resolution while manipulating them for electrophysiological measurements and/or micro-injection. Rather than moving the specimen or the objective for taking Z stacks, adaptive optics are used instead. An SLM will be used for rapid
DeepSIM is the first upright 3D-SIM microscope in the world, uniquely allowing samples to be imaged at super-resolution while manipulating them for electrophysiological measurements and/or micro-injection. Rather than moving the specimen or the objective for taking Z stacks, adaptive optics are used instead. An SLM will be used for rapid production of stripes for live 3D-SIM and for additional aberration corrections.The adaptive optics will allow us to extend the depth of 3D-SIM far beyond what is possible on commercial instruments. The ability to image deep (~50 microns) while manipulating the specimen will be transformational for many biological projects.
This microscope was developed at Micron by Ian Dobbie, Antonia Göhler, Mick Phillips, Mantas Zaurukas, Nick Hall, Josh Titlow and Richard Parton.
Development at Micron
Illan Davis
Martin Booth
Martin Booth
Biomedical Lead
Principal Investigator
Dept of Biochemistry
In the past my lab has focussed on how mRNA transport and localised translation impacted on Drosophila embryonic axis specification. In recent years we have expanded our interest to include the same basic molecular processes to the Drosophila nervous system, in particular the brain and neuromuscular junction.
In order to achieve our scientific aims we use a wide range of scientific techniques, including advanced imaging techniques as well as an array of genetic and biochemistry-based methods.
email: ilan.davis@bioch.ox.ac.uk
Martin Booth
Martin Booth
Martin Booth
Engineering Lead
Principal Investigator
Dept of Engineering Science
Martin's research centres on the development of adaptive optics to compensate for optical abberations caused by focussing through a specimen. These adaptive optics techniques were originally developed for astronomical research, for stabilising and de-blurring telescope images of stars and satellites. Such images are affected by the optical distortions introduced by turbulence in the Earth's atmosphere. Recent technological developments mean that this technology is now being adapted for more down-to-Earth reasons. This has opened up the possibility of using adaptive optics in these smaller scale applications.
email: martin.booth@eng.ox.ac.uk
Jingyu Wang
Martin Booth
Jingyu Wang
Research Microscope Developer
Jingyu is a postdoctoral microscope developer based in Dynamic Optics Group in Engineering Sciences and Micron in Department of Biochemistry. His interests and expertise are on adaptive optics and 3D super-resolution fluorescence microscope techniques. He is currently working on developing adaptive optics assisted ultra-high resolution 3D imaging for cell and tissue samples using 4Pi-SMS (SMLM) and 3D SIM microscopes.
email: jingyu.wang@eng.ox.ac.uk
Orchid ID: 0000-0002-2856-7602
Danail Stoychev
Danail Stoychev
Danail Stoychev
Danny is a BBSRC iCASE student sponsored by Aurox, an Oxford-based startup company, founded by Martin Booth and colleagues. His doctoral research focuses on developing highly usable high-throughput confocal microscopes with adaptive optical using the Python-Microscope_cockpit software developed in Micron.
Richard Parton
Danail Stoychev
Danail Stoychev
I work in the Davis lab using state-of-the-art and advanced imaging techniques as well as developing improved imaging methods and quantitative image analysis to investigate the molecular mechanism of RNA transport in Drosophila tissues. During my own work and through collaborations I have gained considerable experience with quantitative
I work in the Davis lab using state-of-the-art and advanced imaging techniques as well as developing improved imaging methods and quantitative image analysis to investigate the molecular mechanism of RNA transport in Drosophila tissues. During my own work and through collaborations I have gained considerable experience with quantitative live cell imaging and image analysis applied to a variety of biological systems and questions working in collaboration together with researchers with Mathematics, Physics and Chemistry backgrounds. I have a special interest in dynamic cell activities at the single and multi cell level with respect to the determination of cell and tissue growth and development. In addition to my research projects, part of my role is to provide technical advice and imaging support to other researchers and students.
Ian Dobbie
Danail Stoychev
Ian Dobbie
Lead Technical Director 2007-2020
Ian has over 15 years experience in biological imaging gained in a range of leading academic institutes. He gained a degree in physics and a masters in computer modelling before moving on to do a PhD in muscle mechanics at the Randall Division of Cell and Molecular Biophysics at Kings College London. Sinc
Lead Technical Director 2007-2020
Ian has over 15 years experience in biological imaging gained in a range of leading academic institutes. He gained a degree in physics and a masters in computer modelling before moving on to do a PhD in muscle mechanics at the Randall Division of Cell and Molecular Biophysics at Kings College London. Since then he has been working in imaging with a range of biological systems at number of world class research centres including, Cancer Research UK, Kings College London and The University of Oxford. Over the last 10 years he has specialised in advanced fluorescence microscopy.Ian currently works on a wide range of hardware and software development projects, as well as involvement with Micron's many teaching commitments. Ian is now Research Professor and Director of the Integrated Imaging Center at The John Hopkins University.
Granting agencies that funded our work
Micron Strategic Award
Micron Strategic Award
Micron Strategic Award
The primary source of funding for our cutting-edge imaging research.
Principal Applicant: Ilan Davis
Co-applicants: Jordan Raff, Martin Booth, Yvonne Jones, Christian Eggeling, David Stuart, Kay Grunewald, Neil Brockdorff.
4Pi SMS & Microscopi
Micron Strategic Award
Micron Strategic Award
Funded by the Wellcome Institutional Strategic Support Fund and the John Fell Fund, these two innovative microscopy development projects, the 4Pi SMS & Microscopi, are aimed at very different areas of research.
Nanoscopy Oxford
Micron Strategic Award
Nanoscopy Oxford
Nanoscopy Oxford (NanO), a new initiative funded primarily by the MRC to develop novel super-resolution imaging modalities to address key questions in the field of biomedical research.