Leica Thunder Cell Imager

The Leica THUNDER microscope is part of the Leica Centre of Excellence at Micron. The system is based around an inverted widefield microscope,  enabling you to obtain a clear view of details, even deep within an intact sample, in real time without out-of-focus blur. THUNDER brings you high-speed, multicolor imaging of thin and thick samples with increased temporal resolution. 

Applications

• Fast multi-colour widefield imaging in 2D and 3D
• Navigator software for tile-scanning and multi-position imaging
• THUNDER technology for removal of out of focus blur from widefield images. 

Specifications

• Automated contrast methods and control (DIC, phase contrast, brightfield, and fluorescence)
• Automated Z (focus) control for 3D imaging and autofocus.
• Objectives for Long Working Distance (10x, 20x, 40x working with well plates, unattached samples e.g.)
• Objectives with high NA (light gathering capacity) (slides, samples on coverslips)
• Six position fluorescence filter turret, two positions used for two QUAD cubes (eight colours)
• LED8 Fluorescence LED light source, eight separate wavelengths from 390-747nm
• Fast external filter wheel for clean up of emission wavelength at the camera.
• Hamamatsu Fusion camera for speed, resolution and sensitivity, monochrome for fluorescence
• Quantum stage, triggered for speed, linear encoded for repositioning and ease of sample placement.
• Sample holders for slides, dishes, well plates
• Climate control via on stage incubator, temp for keeping cells happy, CO₂ for maintaining pH, humidity control to keep medium concentration stable.
• Conventional microscope stand enables future upgrades e.g.TIRF for extra applications

Access to Micron User Guides (requires Oxford SSO)