Micron BioImaging Facility
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  • Home
  • About us
    • About the Facility
    • Mission Statement
    • Acknowledgement Policy
    • Alumni
  • Our Technology
    • Microscope Systems
    • Image Analysis
  • Access
    • FAQs
    • Booking
  • Education and Outreach
    • Training Courses
    • SIM Practical Course 2022
    • SM Workshop 2023
  • Research
    • Publications
    • Microscope Development
    • Software Development
  • News and Events
  • Image Gallery

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Leica Thunder

Leica Thunder Live Cell Imager

The Leica THUNDER microscope at Micron is an inverted widefield microscope,  enabling you to obtain a clear view of details, even deep within an intact sample, in real time without out-of-focus blur. THUNDER brings you high-speed, multicolor imaging of thin and thick samples with increased temporal resolution. For access please contact micron@bioch.ox.ac.uk.


Specifications

  • Based on the automated, inverted research stand, the DMi8
  • Can image samples with the camera or down the eyepieces.
  • Automated contrast methods and control (DIC, phase contrast, brightfield, and fluorescence)
  • Automated Z (focus) control for 3D imaging and autofocus.
  • Six position nosepiece for objectives
  • Dry and immersion objectives
  • Objectives for Long Working Distance (10x, 20x, 40x working with well plates, unattached samples e.g.)
  • Objectives with high NA (light gathering capacity) (slides, samples on coverslips)
  • Six position fluorescence filter turret, two positions used for two QUAD cubes (eight colours)
  • LED8 Fluorescence LED light source, eight separate wavelengths from 390-747nm
  • Fast external filter wheel for clean up of emission wavelength at the camera.
  •  K8 sCMOS camera for speed, resolution and sensitivity, monochrome for fluorescence
  • Second camera port for colour imaging too
  • Quantum stage, triggered for speed, linear encoded for repositioning and ease of sample placement.
  • Sample holders for slides, dishes, well plates
  • Climate control via on stage incubator, temp for keeping cells happy, CO₂ for maintaining pH, humidity control to keep medium concentration stable.
  • Conventional microscope stand enables future upgrades e.g.TIRF for extra applications
  • LAS X software for imaging in x and y, z, time, multi-position
  • Navigator software for tile-scanning
  • THUNDER technology for removal of out of focus blur from widefield images to give images closer to confocal level images.


Access to Micron User Guides (requires Oxford SSO)


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