Oxford Nanoimager (ONI)

The Nanoimager is a benchtop super-resolution microscope. The instrument can be used for a number of different applications including single-molecule localisation-based super-resolution for quantitative cellular imaging, TIRF and epifluorescence for diffraction-limited fluorescence imaging, single-molecule FRET for measuring molecular interactions in the 2-10 nm range and single particle tracking PALM in cells. Equipped with focus correction. 

Applications

• Super Resolution Localisation Microscopy: PALM / STORM 2D and 3D
• Single-molecule imaging
• smFRET
• Co-localisation and clustering
• Single-particle tracking

Specifications

• 405 nm (150 mW), 532 nm (1000 mW) and 640 nm (1000 mW) lasers (3 laser system)
• 405 nm (150 mW), 488 nm (1000 mW), 560 (1000 mW) and 640 nm (1000 mW) lasers ( 4 laser system)
• 100x NA1.4 UPlanSApo oil objective
• Hamamatsu ORCA-Flash 4.0 (camera chip split)
• Channel splitter dichroic 640 LP 
• Emission Channel 0 Band 1 498 - 524 nm
• Emission Channel 0 Band 2 550 - 620 nm
• Emission Channel 1 Band 3 685/40 nm
• Temperature Control

Image Acknowledgement 

Following the movement of KDEL receptor 

Endoplasmic reticulum KDEL receptor is followed through time using live PALM on the ONI microscope. Each track represent a different trajectory of each receptor over time. 

Andreas Gerondopoulos and Danny Gold,  Barr Group, Department of Biochemistry. 

Access to Micron User Guides (requires Oxford SSO)