ONI Nanoimager

The Nanoimager is a benchtop super-resolution microscope. The instrument can be used for a number of different applications including single-molecule localisation-based super-resolution for quantitative cellular imaging, TIRF and epifluorescence for diffraction-limited fluorescence imaging, single-molecule FRET for measuring molecular interactions in the 2-10 nm range and single particle tracking PALM in cells. Equipped with focus correction. For access please contact micron@bioch.ox.ac.uk.

Applications

• Super Resolution Localisation Microscopy: PALM / STORM 2D and 3D
• Single-molecule imaging
• smFRET
• Co-localisation and clustering
• Single-particle tracking

Specifications

• 405 nm (150 mW), 532 nm (1000 mW) and 640 nm (1000 mW) lasers (3 laser system)
• 405 nm (150 mW), 488 nm (1000 mW), 560 (1000 mW) and 640 nm (1000 mW) lasers ( 4 laser system)
• 100x NA1.4 UPlanSApo oil objective
• Hamamatsu ORCA-Flash 4.0 (camera chip split)
• Channel splitter dichroic 640 LP 
• Emission Channel 0 Band 1 498 - 524 nm
• Emission Channel 0 Band 2 550 - 620 nm
• Emission Channel 1 Band 3 685/40 nm
• Temperature Control

Image Acknowledgement 

Visualisation of Endoplasmic Reticulum using dSTORM (above)

Adopting cutting-edge 3D dSTORM imaging technology, using the ONI Nanoimager, to elucidate cellular structure and determine particle localisations. 

Andreas Gerondopoulos and Danny Gold,  Barr Group, Department of Biochemistry. 

Following the movement of KDEL receptor (below)

Endoplasmic reticulum KDEL receptor is followed through time using live PALM on the ONI microscope. Each track represent a different trajectory of each receptor over time. 

Andreas Gerondopoulos and Danny Gold,  Barr Group, Department of Biochemistry. 

Access to Micron User Guides (requires Oxford SSO)