Micron BioImaging Facility
Micron BioImaging Facility
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      • SIM Practical Course 2022
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  • Home
  • About us
    • About the Facility
    • Mission Statement
    • Acknowledgement Policy
    • Alumni
  • Our Technology
    • Microscope Systems
    • Image Analysis
  • Access
    • FAQs
    • Booking
  • Education and Outreach
    • Training Courses
    • SIM Practical Course 2022
    • SM Workshop 2023
  • Research
    • Publications
    • Microscope Development
    • Software Development
  • News and Events
  • Image Gallery

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Leica Stellaris Confocal

A high-speed, laser scanning confocal with fluorescence lifetime and super-resolution capabilities. For access please contact micron@bioch.ox.ac.uk.


Specifications


  • DMi8 CS Premium, inverted motorised microscope stand
  • Epi-fluorescence lamp and filters for eye piece viewing 
  • Transmitted light detector for brightfield/DIC/POL 
  • Motorized DIC
  • Lasers: 405 nm and White Light Laser, 485 nm – 790 nm
  • 5 confocal fluorescence detectors (HyD S: photon counting, detection up to 850
    nm)
  • 8 kHz Tandem scanner 
  • FRAP, FRET capabilities
  • Super Z galvo stage (inserts: universal, petri dish, multi-well) 
  • Adaptive Focus Control (Hardware Autofocus) 
  • TauSense tools for lifetime-based contrast, gating, separation, FRET 
  • LAS X Navigator for multi-position, multi-region and overview imaging 
  • Navigator Expert and Autonomous Microscopy (for AI based rare event detection) 
  • Lightning Super Resolution (improves resolution to 120 nm in xy, 200 nm in z) 
  • Aivia Go image analysis 
  • Objectives – 5x, 10x, 20x, 20x multi-immersion, 63x oil 


Access to Micron User Guides (requires Oxford SSO)

User Guides

White Light Laser

How the White Light Laser works

Confocal imaging of a kidney section

3D rendered movie of a kidney section captured using a Leica confocal microscope.  Alexa Fluor® 488 wheat germ agglutinin is a fluorescent lectin that labels the glomeruli and convoluted tubules (cyan). Actin located in glomeruli and the brush border are stained with Alexa Fluor® 568 phalloidin (magenta), nuclei are stained with the  DNA stain DAPI (grey).




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